Human HPRT1 qPCR primer set for hypoxanthine phosphoribosyltransferase 1 (HGPRT)

SYBR Green qPCR primers for detecting human HPRT1 (hypoxanthine phosphoribosyltransferase 1) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Human HPRT1 qPCR Primer Set – hypoxanthine phosphoribosyltransferase 1

Also known as: HGPRT, HPRT

About this gene: According to NCBI Gene, the HPRT1 protein is a transferase that catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate by transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout. HPRT1 is widely used as a reference (housekeeping) gene for normalizing qPCR data from human cells and tissues. Many expression studies include HPRT1 alongside or instead of other common reference genes. It is also measured directly when researchers are studying purine salvage, nucleotide metabolism, or HPRT1-related disorders.

Gene details: NCBI Gene ID: 3251; HGNC ID: HGNC:5157; UniProt: P00492; RefSeq (MANE Select): NM_000194.3; Chromosome location: Xq26.2-q26.3

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Human PCR Primer Sets, or profile HPRT1 alongside related genes with our Housekeeping Gene Primer Sets.

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