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Human GAPDH qPCR primer set for glyceraldehyde-3-phosphate dehydrogenase

GAPDH (Human)
Item# VHPS-3541T
$24.95

Product Description

SYBR Green qPCR primers for detecting human GAPDH (glyceraldehyde-3-phosphate dehydrogenase) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Human GAPDH qPCR Primer Set – glyceraldehyde-3-phosphate dehydrogenase

Also known as: G3PD, GAPD, HEL-S-162eP

About this gene: According to NCBI Gene, GAPDH encodes a member of the glyceraldehyde-3-phosphate dehydrogenase family and is a moonlighting protein, meaning the product can carry out mechanistically distinct functions. Its canonical role is an important energy-yielding step in carbohydrate metabolism: the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). NCBI also reports nuclear uracil DNA glycosylase activity. Studies of a similar protein in mouse have assigned further roles, including nitrosylation of nuclear proteins, regulation of mRNA stability, and acting as a transferrin receptor on the macrophage cell surface. Alternative splicing produces multiple transcript variants, and many related pseudogenes are present in the human genome. In the lab, GAPDH is commonly used as a reference (housekeeping) gene to normalize qPCR data. It is also a direct target when researchers are studying glycolysis or these non-canonical GAPDH functions.

Gene details: NCBI Gene ID: 2597; HGNC ID: HGNC:4141; UniProt: P04406; RefSeq (MANE Select): NM_002046.7; Chromosome location: 12p13.31

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Human GAPDH qPCR: representative amplification plot and melt curve with a single peak, SYBR Green.
Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Human PCR Primer Sets, or profile GAPDH alongside related genes with our Housekeeping Gene Primer Sets.



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