Human CDKN1A qPCR primer set for cyclin dependent kinase inhibitor 1A (p21)

SYBR Green qPCR primers for detecting human CDKN1A (cyclin dependent kinase inhibitor 1A, also called p21) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Human CDKN1A qPCR Primer Set – cyclin dependent kinase inhibitor 1A

Also known as: CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1

About this gene: NCBI Gene states that CDKN1A encodes a potent cyclin-dependent kinase inhibitor. The protein binds to and inhibits cyclin-CDK2 or cyclin-CDK4 complexes and thus regulates cell cycle progression at G1. Its expression is tightly controlled by the tumor suppressor protein p53, through which it mediates p53-dependent G1 arrest in response to a variety of stress stimuli. The protein can also interact with proliferating cell nuclear antigen (PCNA) and plays a regulatory role in S-phase DNA replication and DNA damage repair. NCBI reports that the protein can be cleaved by CASP3-like caspases, leading to activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found. Human CDKN1A is a standard qPCR target in studies of the p53 pathway, cell-cycle arrest, senescence, DNA damage, and apoptosis, and it is often measured alongside TP53.

Gene details: NCBI Gene ID: 1026; HGNC ID: HGNC:1784; UniProt: P38936; RefSeq (MANE Select): NM_000389.5; Chromosome location: 6p21.2

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Human PCR Primer Sets.

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