Human ACTB qPCR primer set for actin beta (beta-actin)

SYBR Green qPCR primers for detecting human ACTB (actin beta, also called beta-actin) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Human ACTB qPCR Primer Set – actin beta

Also known as: BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8

About this gene: NCBI Gene states that ACTB encodes one of six different actin proteins. Actins are highly conserved and are involved in cell motility, structure, integrity, and intercellular signaling. The ACTB protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance. Numerous ACTB pseudogenes have been identified in the human genome. Because beta-actin is ubiquitously expressed and abundant, ACTB is widely used as a reference (housekeeping) gene for normalizing qPCR and other expression assays. Researchers also measure ACTB when they are studying the cytoskeleton, cell motility, or related structural processes.

Gene details: NCBI Gene ID: 60; HGNC ID: HGNC:132; UniProt: P60709; RefSeq (MANE Select): NM_001101.5; Chromosome location: 7p22.1

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Human PCR Primer Sets, or profile ACTB alongside related genes with our Housekeeping Gene Primer Sets.

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