Human Breast Cancer Signaling Primer Library

Contains 88 primer sets directed against breast cancer signaling genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ABL1 c-abl oncogene 1, non-receptor tyrosine kinase

AHR aryl hydrocarbon receptor

AKT1 v-akt murine thymoma viral oncogene homolog 1

ANXA1 annexin A1

ARAF v-raf murine sarcoma 3611 viral oncogene homolog

ATM ataxia telangiectasia mutated

ATR ataxia telangiectasia and Rad3 related

BACH1 BTB and CNC homology 1, basic leucine zipper transcription factor 1

BAD BCL2-associated agonist of cell death

BARD1 BRCA1 associated RING domain 1

BCL2 B-cell CLL/lymphoma 2

BLM Bloom syndrome, RecQ helicase-like

BMPR1A bone morphogenetic protein receptor, type IA

BMPR2 bone morphogenetic protein receptor, type II (serine/threonine kinase)

BRAF v-raf murine sarcoma viral oncogene homolog B1

BRCA1 breast cancer 1, early onset

BRCA2 breast cancer 2, early onset

CDH1 cadherin 1, type 1, E-cadherin (epithelial)

CDK2 cyclin-dependent kinase 2

CDK4 cyclin-dependent kinase 4

CDK7 cyclin-dependent kinase 7

CERK ceramide kinase

CREB1 cAMP responsive element binding protein 1

CSNK1D casein kinase 1, delta

DAG1 dystroglycan 1 (dystrophin-associated glycoprotein 1)

DCAKD dephospho-CoA kinase domain containing

DHTKD1 dehydrogenase E1 and transketolase domain containing 1

EDAR ectodysplasin A receptor

EGFR epidermal growth factor receptor

EP300 E1A binding protein p300

ERRB2 V-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

ESR1 estrogen receptor 1

FADD Fas (TNFRSF6)-associated via death domain

FER fer (fps/fes related) tyrosine kinase

FOXO1 forkhead box O1

GDI1 GDP dissociation inhibitor 1

GRN granulin

GSK3A glycogen synthase kinase 3 alpha

HDAC1 histone deacetylase 1

IMPA1 inositol(myo)-1(or 4)-monophosphatase 1

IRS1 insulin receptor substrate 1

ITPKC inositol-trisphosphate 3-kinase C

JUN jun proto-oncogene

KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

MAPK1 mitogen-activated protein kinase 1

MAX MYC associated factor X

MDM2 Mdm2 p53 binding protein homolog (mouse)

MMP1 matrix metallopeptidase 1 (interstitial collagenase)

MSH2 mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)

MSH6 mutS homolog 6 (E. coli)

MYC V-myc myelocytomatosis viral oncogene homolog (avian)

MYT1 myelin transcription factor 1

NAB1 NGFI-A binding protein 1 (EGR1 binding protein 1)

NF1 neurofibromin 1

NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

NOXA1 NADPH oxidase activator 1

NUP85 nucleoporin 85kDa

PAK1 p21 protein (Cdc42/Rac)-activated kinase 1

PIGR polymeric immunoglobulin receptor

PLK1 polo-like kinase 1

PLK3 polo-like kinase 3

PML promyelocytic leukemia

PTEN phosphatase and tensin homolog

RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

RAD50 RAD50 homolog (S. cerevisiae)

RAD51 RAD51 homolog (S. cerevisiae)

RALA v-ral simian leukemia viral oncogene homolog A (ras related)

RALGAPA1 Ral GTPase activating protein, alpha subunit 1 (catalytic)

RAP1A RAP1A, member of RAS oncogene family

RASGEF1A RasGEF domain family, member 1A

RASGRP3 RAS guanyl releasing protein 3 (calcium and DAG- regulated)

RHEB Ras homolog enriched in brain

RHO rhodopsin

RPP38 ribonuclease P/MRP 38kDa subunit

SELK selenoprotein K

SIRT1 sirtuin 1

SMAD1 SMAD family member 1

SMAD2 SMAD family member 2

SMEK1 SMEK homolog 1, suppressor of mek1 (Dictyostelium)

SMEK2 SMEK homolog 2, suppressor of mek1 (Dictyostelium)

STAT1 signal transducer and activator of transcription 1, 91kDa

TAB1 TGF-beta activated kinase 1/MAP3K7 binding protein 1

TPR translocated promoter region (to activated MET oncogene)

TRADD TNFRSF1A-associated via death domain

TSC1 tuberous sclerosis 1

TSC2 tuberous sclerosis 2

VEGFA vascular endothelial growth factor A

WEE1 WEE1 homolog (S. pombe)

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPDH Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK1 Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a