Drug detoxication proceeds through coordinated phase I oxidation, phase II conjugation and phase III transport. Phase II conjugating enzymes, including glutathione S-transferases (GSTs), UDP-glucuronosyltransferases (UGTs), sulfotransferases (SULTs) and N-acetyltransferases (NATs), make drugs and toxins more water soluble, while antioxidant and detoxifying enzymes regulated by NRF2 protect cells from reactive metabolites. Variation in the expression of these genes influences drug response, toxicity and susceptibility to environmental carcinogens.
Contains 88 primer sets directed against drug detoxication genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix GENE LIST: AANAT Arylalkylamine N-acetyltransferase ACSL1 Acyl-CoA synthetase long-chain family member 1 ACSL3 Acyl-CoA synthetase long-chain family member 3 ACSL4 Acyl-CoA synthetase long-chain family member 4 ACSL5 Acyl-CoA synthetase long-chain family member 5 ACSL6 Acyl-CoA synthetase long-chain family member 6 ACSM1 Acyl-CoA synthetase medium-chain family member 1 ACSM2B Acyl-CoA synthetase medium-chain family member 2B ACSM3 Acyl-CoA synthetase medium-chain family member 3 AGXT Alanine-glyoxylate aminotransferase AS3MT Arsenic (+3 oxidation state) methyltransferase ASMT Acetylserotonin O-methyltransferase BAAT Bile acid Coenzyme A: amino acid N-acyltransferase (glycine N-choloyltransferase) CCBL1 Cysteine conjugate-beta lyase, cytoplasmic CES1 Carboxylesterase 1 (monocyte/macrophage serine esterase 1) CES2 Carboxylesterase 2 (intestine, liver) CES3 Carboxylesterase 3 CES4A Carboxylesterase 4A CES5A Carboxylesterase 5A CES7 Carboxylesterase 7 COMT Catechol-O-methyltransferase DDOST Dolichyl-diphosphooligosaccharide-protein glycosyltransferase EPHX1 Epoxide hydrolase 1, microsomal (xenobiotic) EPHX2 Epoxide hydrolase 2, cytoplasmic GAMT Guanidinoacetate N-methyltransferase GLYAT Glycine-N-acyltransferase GNMT Glycine N-methyltransferase GSTA1 Glutathione S-transferase alpha 1 GSTA3 Glutathione S-transferase alpha 3 GSTA4 Glutathione S-transferase alpha 4 GSTA5 Glutathione S-transferase alpha 5 GSTK1 Glutathione S-transferase kappa 1 GSTM2 Glutathione S-transferase mu 2 (muscle) GSTM3 Glutathione S-transferase mu 3 (brain) GSTM4 Glutathione S-transferase mu 4 GSTM5 Glutathione S-transferase mu 5 GSTO1 Glutathione S-transferase omega 1 GSTO2 Glutathione S-transferase omega 2 GSTP1 Glutathione S-transferase pi 1 GSTT1 Glutathione S-transferase theta 1 GSTT2 Glutathione S-transferase theta 2 HNMT Histamine N-methyltransferase INMT Indolethylamine N-methyltransferase MGST1 Microsomal glutathione S-transferase 1 MGST2 Microsomal glutathione S-transferase 2 MGST3 Microsomal glutathione S-transferase 3 NAT1 N-acetyltransferase 1 (arylamine N- acetyltransferase) NAT2 N-acetyltransferase 2 (arylamine N- acetyltransferase) NAT5 N-acetyltransferase 5 (GCN5-related, putative) NNMT Nicotinamide N-methyltransferase NQO1 NAD(P)H dehydrogenase, quinone 1 NQO2 NAD(P)H dehydrogenase, quinone 2 PNMT Phenylethanolamine N-methyltransferase PTGES Prostaglandin E synthase SAT1 Spermidine/spermine N1-acetyltransferase 1 SULT1A1 Sulfotransferase family, cytosolic, 1A, phenol- preferring, member 1 SULT1A2 Sulfotransferase family, cytosolic, 1A, phenol- preferring, member 2 SULT1A3 Sulfotransferase family, cytosolic, 1A, phenol- preferring, member 3 SULT1B1 Sulfotransferase family, cytosolic, 1B, member 1 SULT1C1 Sulfotransferase family, cytosolic, 1C, member 2 SULT1C2 Sulfotransferase family, cytosolic, 1C, member 2 SULT1C3 Sulfotransferase family, cytosolic, 1C, member 3 SULT1E1 Sulfotransferase family 1E, estrogen-preferring, member 1 SULT2A1 Sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone (DHEA)-preferring, member 1 SULT2B1 Sulfotransferase family, cytosolic, 2B, member 1 SULT4A1 Sulfotransferase family 4A, member 1 SULT6B1 Sulfotransferase family, cytosolic, 6B, member 1 TPMT Thiopurine S-methyltransferase TST Thiosulfate sulfurtransferase (rhodanese) UGT1A1 UDP glucuronosyltransferase 1 family, polypeptide A1 UGT1A10 UDP glucuronosyltransferase 1 family, polypeptide A10 UGT1A3 UDP glucuronosyltransferase 1 family, polypeptide A3 UGT1A4 UDP glucuronosyltransferase 1 family, polypeptide A4 UGT1A5 UDP glucuronosyltransferase 1 family, polypeptide A5 UGT1A6 UDP glucuronosyltransferase 1 family, polypeptide A6 UGT1A7 UDP glucuronosyltransferase 1 family, polypeptide A7 UGT1A8 UDP glucuronosyltransferase 1 family, polypeptide A8 UGT1A9 UDP glucuronosyltransferase 1 family, polypeptide A9 UGT2A1 UDP glucuronosyltransferase 2 family, polypeptide A1 UGT2A3 UDP glucuronosyltransferase 2 family, polypeptide A3 UGT2B10 UDP glucuronosyltransferase 2 family, polypeptide B10 UGT2B17 UDP glucuronosyltransferase 2 family, polypeptide B17 UGT2B28 UDP glucuronosyltransferase 2 family, polypeptide B28 UGT2B4 UDP glucuronosyltransferase 2 family, polypeptide B4 UGT2B7 UDP glucuronosyltransferase 2 family, polypeptide B7 UGT3A1 UDP glycosyltransferase 3 family, polypeptide A1 UGT8 UDP glycosyltransferase 8 XDH Xanthine dehydrogenase ACTB Actin, beta B2M Beta-2-microglobulin GAPDH Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 PGK1 Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $595.00 |