RealTimePrimers.com
Validated PCR Primer Sets (1-855-PRIMERS)Perform PCR Arrays for less than $2.00?
Library of targeted real time PCR primer sets Perform up to 200 PCR Arrays! That's $2 per array! (Based on a 10 ul reaction volume) Flexibility to design one's own experiments Microplate containing 88 targeted plus 8 housekeeping gene primer sets Note: Primer sequences unavailable for primer libraries |
Quantitative Real Time PCR Primer Sets - ONLY $24.95
Our goal is to provide a cost-effective approach to validate microarray data and quantitate gene expression using SYBR green-based real time PCR assays
Perform up to 1000 assays
Detailed information on optimal reaction conditions provided
Primer sequences provided!
SELECTED PUBLICATIONS:
Abdel-Hakeem, AK, Henry, TQ, Magee, TR et al. (2008) Mechanisms of impaired nephrogenesis with fetal growth restriction: altered renal transcription and growth factor expression. Am J Obstet Gynecol.199, 252 e1-e7.
Pedroja BS, Kang LE, Imas AO, Carmeliet P, Bernstein AM. (2009) Plasminogen Activator Inhibitor-1 Regulates Integrin ávâ3 Expression and Autocrine Transforming Growth Factor â Signaling. J Biol Chem. 284, 20708-20717.
Davis, AM, Mao, J, Naz, B., Koh., JA, Rosenfeld, CS. (2008) Comparative effects of estradiol, methyl-piperidino-pyrazole, raloxifene, and ICI 182 780 on gene expression in the murine uterus. Journal of Molecular Endocrinology 41, 205-217.
Bubier JA, Sproule TJ, Foreman O, Spolski R, Shaffer DJ, Morse HC 3rd, Leonard WJ, Roopenian DC. (2009) A critical role for IL-21 receptor signaling in the pathogenesis of systemic lupus erythematosus in BXSB-Yaa mice. Proc. Natl. Acad. Sci USA 106, 1518-1523.
Cheng Y, Kim JW, Liu W, Dunn TA, Luo J, Loza MJ, Kim ST, Zheng SL, Xu J, Isaacs WB, Chang BL (2009) Genetic and epigenetic inactivation of TNFRSF10C in human prostate cancer. Prostate 69, 327-335.
Liao D, Luo Y, Markowitz D, Xiang R, Reisfeld RA. (2009) Cancer associated fibroblasts promote tumor growth and metastasis by modulating the tumor immune microenvironment in a 4T1 murine breast cancer model. PloS One 23, e7965.
Desai S, Bolick SC, Maurin M, Wright, KL (2009) PU.1 regulates positive regulatory domain I-binding factor 1/Blimp-1 transcription in lymphoma cells. J. Immunol 183, 5778-5787.
Lange J, Sapozhnikova A, Lu C, Hu D, Li X, Miclau T 3rd, Marcucio RS. (2010) Action of IL-1beta during fracture healing.J. Orthop. Res. 28, 778-784.
Avdoshina V, Biggio F, Palchik G, Campbell LA, Mocchetti I. (2010) Morphine induces the release of CCL5 from astrocytes: potential neuroprotective mechanism against the HIV protein gp120. Glia 58, 1630-1639.
Eyerich K, Foerster S, Rombold S, Seidl HP, Behrendt H, Hofmann H, Ring J, Traidl-Hoffmann C. (2008) Patients with chronic mucocutaneous candidiasis exhibit reduced production of Th17-associated cytokines IL-17 and IL-22. J. Invest. Dermatol. 128, 2640-2645.
Sengupta S, Mantha AK, Mitra S, Bhakat KK. (2010) Human AP endonuclease (APE1/Ref-1) and its acetylation regulate YB-1-p300 recruitment and RNA polymerase II loading in the drug-induced activation of multidrug resistance gene MDR1. Oncogene [Epub ahead of print]
Akers SM, O'Leary HA, Minnear FL, Craig MD, Vos JA, Coad JE, Gibson LF. (2010) VE-cadherin and PECAM-1 enhance ALL migration across brain microvascular endothelial cell monolayers. Exp. Hematol. 38, 733-743.
Hastings RH, Montgrain PR, Quintana R, Rascon Y, Deftos LJ, Healy E. (2009) Cell cycle actions of parathyroid hormone-related protein in non-small cell lung carcinoma. Am J Physiol Lung Cell Mol Physiol. 297, L578-585.
Sudarshan SR, Schlegel R, Liu X. (2010) The HPV-16 E5 protein represses expression of stress pathway genes XBP-1 and COX-2 in genital keratinocytes. Biochem Biophys Res Commun.399, 617-622.
Cramer C, Freisinger E, Jones RK, Slakey DP, Dupin CL, Newsome ER, Alt EU, Izadpanah R. (2010) Persistent High Glucose Concentrations Alter the Regenerative Potential of Mesenchymal Stem Cells. Stem Cells Dev. [Epub ahead of print]
Alexanian A, Rufanova VA, Miller B, Flasch A, Roman RJ, Sorokin A. (2009) Down-regulation of 20-HETE synthesis and signaling inhibits renal adenocarcinoma cell proliferation and tumor growth. Anticancer Res. 29, 3819-3824.
Hernandez L, Hsu SC, Davidson B, Birrer MJ, Kohn EC, Annunziata CM. (2010) Activation of NF-kappaB signaling by inhibitor of NF-kappaB kinase beta increases aggressiveness of ovarian cancer. CancerRes. 0, 4005-4014.
Zhang Y, Kohan DE, Nelson RD, Carlson NG, Kishore BK. (2009) Potential involvement of P2Y2 receptor in diuresis of postobstructive uropathy in rats. Am J Physiol Renal Physiol. 298, F634-42.
Perform up to 1000 assays
Detailed information on optimal reaction conditions provided
Primer sequences provided!
SELECTED PUBLICATIONS:
Abdel-Hakeem, AK, Henry, TQ, Magee, TR et al. (2008) Mechanisms of impaired nephrogenesis with fetal growth restriction: altered renal transcription and growth factor expression. Am J Obstet Gynecol.199, 252 e1-e7.
Pedroja BS, Kang LE, Imas AO, Carmeliet P, Bernstein AM. (2009) Plasminogen Activator Inhibitor-1 Regulates Integrin ávâ3 Expression and Autocrine Transforming Growth Factor â Signaling. J Biol Chem. 284, 20708-20717.
Davis, AM, Mao, J, Naz, B., Koh., JA, Rosenfeld, CS. (2008) Comparative effects of estradiol, methyl-piperidino-pyrazole, raloxifene, and ICI 182 780 on gene expression in the murine uterus. Journal of Molecular Endocrinology 41, 205-217.
Bubier JA, Sproule TJ, Foreman O, Spolski R, Shaffer DJ, Morse HC 3rd, Leonard WJ, Roopenian DC. (2009) A critical role for IL-21 receptor signaling in the pathogenesis of systemic lupus erythematosus in BXSB-Yaa mice. Proc. Natl. Acad. Sci USA 106, 1518-1523.
Cheng Y, Kim JW, Liu W, Dunn TA, Luo J, Loza MJ, Kim ST, Zheng SL, Xu J, Isaacs WB, Chang BL (2009) Genetic and epigenetic inactivation of TNFRSF10C in human prostate cancer. Prostate 69, 327-335.
Liao D, Luo Y, Markowitz D, Xiang R, Reisfeld RA. (2009) Cancer associated fibroblasts promote tumor growth and metastasis by modulating the tumor immune microenvironment in a 4T1 murine breast cancer model. PloS One 23, e7965.
Desai S, Bolick SC, Maurin M, Wright, KL (2009) PU.1 regulates positive regulatory domain I-binding factor 1/Blimp-1 transcription in lymphoma cells. J. Immunol 183, 5778-5787.
Lange J, Sapozhnikova A, Lu C, Hu D, Li X, Miclau T 3rd, Marcucio RS. (2010) Action of IL-1beta during fracture healing.J. Orthop. Res. 28, 778-784.
Avdoshina V, Biggio F, Palchik G, Campbell LA, Mocchetti I. (2010) Morphine induces the release of CCL5 from astrocytes: potential neuroprotective mechanism against the HIV protein gp120. Glia 58, 1630-1639.
Eyerich K, Foerster S, Rombold S, Seidl HP, Behrendt H, Hofmann H, Ring J, Traidl-Hoffmann C. (2008) Patients with chronic mucocutaneous candidiasis exhibit reduced production of Th17-associated cytokines IL-17 and IL-22. J. Invest. Dermatol. 128, 2640-2645.
Sengupta S, Mantha AK, Mitra S, Bhakat KK. (2010) Human AP endonuclease (APE1/Ref-1) and its acetylation regulate YB-1-p300 recruitment and RNA polymerase II loading in the drug-induced activation of multidrug resistance gene MDR1. Oncogene [Epub ahead of print]
Akers SM, O'Leary HA, Minnear FL, Craig MD, Vos JA, Coad JE, Gibson LF. (2010) VE-cadherin and PECAM-1 enhance ALL migration across brain microvascular endothelial cell monolayers. Exp. Hematol. 38, 733-743.
Hastings RH, Montgrain PR, Quintana R, Rascon Y, Deftos LJ, Healy E. (2009) Cell cycle actions of parathyroid hormone-related protein in non-small cell lung carcinoma. Am J Physiol Lung Cell Mol Physiol. 297, L578-585.
Sudarshan SR, Schlegel R, Liu X. (2010) The HPV-16 E5 protein represses expression of stress pathway genes XBP-1 and COX-2 in genital keratinocytes. Biochem Biophys Res Commun.399, 617-622.
Cramer C, Freisinger E, Jones RK, Slakey DP, Dupin CL, Newsome ER, Alt EU, Izadpanah R. (2010) Persistent High Glucose Concentrations Alter the Regenerative Potential of Mesenchymal Stem Cells. Stem Cells Dev. [Epub ahead of print]
Alexanian A, Rufanova VA, Miller B, Flasch A, Roman RJ, Sorokin A. (2009) Down-regulation of 20-HETE synthesis and signaling inhibits renal adenocarcinoma cell proliferation and tumor growth. Anticancer Res. 29, 3819-3824.
Hernandez L, Hsu SC, Davidson B, Birrer MJ, Kohn EC, Annunziata CM. (2010) Activation of NF-kappaB signaling by inhibitor of NF-kappaB kinase beta increases aggressiveness of ovarian cancer. CancerRes. 0, 4005-4014.
Zhang Y, Kohan DE, Nelson RD, Carlson NG, Kishore BK. (2009) Potential involvement of P2Y2 receptor in diuresis of postobstructive uropathy in rats. Am J Physiol Renal Physiol. 298, F634-42.