Human Hypoxia Signaling Primer Library

Contains 88 primer sets directed against hypoxia signaling genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

ADM Adrenomedullin

AGPAT2 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta)

ANGPTL4 Angiopoietin-like 4

ARNT aryl hydrocarbon receptor nuclear translocator

ARNT2 Aryl-hydrocarbon receptor nuclear translocator 2

ATP1B1 ATPase, Na+/K+ transporting, beta 1 polypeptide

BAX BCL2-associated X protein

BIRC5 Baculoviral IAP repeat-containing 5

BNIP3L BCL2/adenovirus E1B 19kDa interacting protein 3- like

CA1 Carbonic anhydrase I

CAT Catalase

CDC42 Cell division cycle 42 (GTP binding protein, 25kDa)

CHGA Chromogranin A (parathyroid secretory protein 1)

COL1A1 Collagen, type I, alpha 1

CREBBP CREB binding protein

CSTB Cystatin B (stefin B)

CUL2 cullin 2

CXCR4 chemokine (C-X-C motif) receptor 4

CYGB Cytoglobin

DDIT4 DNA-damage-inducible transcript 4

DDIT4L DNA-damage-inducible transcript 4-like

ECE1 Endothelin converting enzyme 1

EDN1 endothelin 1

EEF1A1 Eukaryotic translation elongation factor 1 alpha 1

EGLN1 egl nine homolog 1 (C. elegans)

EGLN2 egl nine homolog 2 (C. elegans)

EGLN3 egl nine homolog 3 (C. elegans)

ENO1 Enolase 1, (alpha)

EP300 E1A binding protein p300

EPAS1 Endothelial PAS domain protein 1

EPO Erythropoietin

FRAP1 Mechanistic target of rapamycin (serine/threonine kinase) (MTOR)

GNA11 Guanine nucleotide binding protein (G protein), alpha 11 (Gq class)

GPI Glucose phosphate isomerase

GPX1 Glutathione peroxidase 1

HBB Hemoglobin, beta

HIF1A Hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

HIF1AN Hypoxia inducible factor 1, alpha subunit inhibitor

HIF3A Hypoxia inducible factor 3, alpha subunit

HIG2

HK2 Hexokinase 2

HMOX1 Heme oxygenase (decycling) 1

HYOU1 Hypoxia up-regulated 1

ICAM1 intercellular adhesion molecule 1

IGF2 Insulin-like growth factor 2 (somatomedin A)

IGFBP1 Insulin-like growth factor binding protein 1

IL1A Interleukin 1, alpha

IL6 Interleukin 6 (interferon, beta 2)

IL6ST Interleukin 6 signal transducer (gp130, oncostatin M receptor)

ING4 inhibitor of growth family, member 4

KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

LDHA lactate dehydrogenase A

LEP Leptin

MB myoglobin

MT3 Metallothionein 3

MYBL2 V-myb myeloblastosis viral oncogene homolog (avian)-like 2

NOS1 nitric oxide synthase 1 (neuronal)

NOS2 Nitric oxide synthase 2, inducible

NOS3 nitric oxide synthase 3 (endothelial cell)

NOTCH1 Notch homolog 1, translocation-associated (Drosophila)

PDIA2 Protein disulfide isomerase family A, member 2

PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide

PLAU Plasminogen activator, urokinase

POU5F1 POU class 5 homeobox 1

PPARA Peroxisome proliferator-activated receptor alpha

PPP2CB Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform

PRKAA1 Protein kinase, AMP-activated, alpha 1 catalytic subunit

PRKAA2 protein kinase, AMP-activated, alpha 2 catalytic subunit

PRPF40A PRP40 pre-mRNA processing factor 40 homolog A (S. cerevisiae)

PSMB3 Proteasome (prosome, macropain) subunit, beta type, 3

PTEN phosphatase and tensin homolog

PTX3 Pentraxin-related gene, rapidly induced by IL-1 beta

RARA Retinoic acid receptor, alpha

RBX1 ring-box 1, E3 ubiquitin protein ligase

SLC2A1 Solute carrier family 2 (facilitated glucose transporter), member 1

SLC2A4 Solute carrier family 2 (facilitated glucose transporter), member 4

SLC2A8 solute carrier family 2 (facilitated glucose transporter), member 8

SOD3 superoxide dismutase 3, extracellular

SSSCA1 Sjogren syndrome/scleroderma autoantigen 1

SUMO2 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)

TGFBR2 transforming growth factor, beta receptor II (70/80kDa)

TH Tyrosine hydroxylase

TP53 tumor protein p53

TST Thiosulfate sulfurtransferase (rhodanese)

TUBA4A Tubulin, alpha 4a

UCP2 Uncoupling protein 2 (mitochondrial, proton carrier)

VCAM1 Vascular cell adhesion molecule 1

VEGFA vascular endothelial growth factor A

ACTB Actin, beta

B2M Beta-2-microglobulin

GAPDH Glyceraldehyde-3-phosphate dehydrogenase

GUSB Glucuronidase, beta

HPRT1 Hypoxanthine phosphoribosyltransferase 1

PGK1 Phosphoglycerate kinase 1

PPIA Peptidylprolyl isomerase A

RPL13A Ribosomal protein L13a